![Primers (forward and reverse) are synthetic oligonucleotides of 17-30 nucleotides. They are complementary to the sequence present on the desired DNA segment. Why? Primers (forward and reverse) are synthetic oligonucleotides of 17-30 nucleotides. They are complementary to the sequence present on the desired DNA segment. Why?](https://www.vedantu.com/question-sets/13e8f686-b5c3-4ba6-a6ce-c88ec8deb2ed561498777334811137.png)
Primers (forward and reverse) are synthetic oligonucleotides of 17-30 nucleotides. They are complementary to the sequence present on the desired DNA segment. Why?
![genetics - Is it possible to use two forward primer and one reverse primer? - Biology Stack Exchange genetics - Is it possible to use two forward primer and one reverse primer? - Biology Stack Exchange](https://i.stack.imgur.com/zlIoH.jpg)
genetics - Is it possible to use two forward primer and one reverse primer? - Biology Stack Exchange
![Phases of competitor DNA construction. F. forward primer obtained in... | Download Scientific Diagram Phases of competitor DNA construction. F. forward primer obtained in... | Download Scientific Diagram](https://www.researchgate.net/publication/266284183/figure/fig1/AS:641700728942595@1530004784379/Phases-of-competitor-DNA-construction-F-forward-primer-obtained-in-Neilan-et-al.png)
Phases of competitor DNA construction. F. forward primer obtained in... | Download Scientific Diagram
![Highly specific real-time quantification of diverse microRNAs in human samples using universal primer set frame - ScienceDirect Highly specific real-time quantification of diverse microRNAs in human samples using universal primer set frame - ScienceDirect](https://ars.els-cdn.com/content/image/1-s2.0-S0003269717304979-fx1.jpg)
Highly specific real-time quantification of diverse microRNAs in human samples using universal primer set frame - ScienceDirect
![Real‐time PCR (qPCR) primer design using free online software - Thornton - 2011 - Biochemistry and Molecular Biology Education - Wiley Online Library Real‐time PCR (qPCR) primer design using free online software - Thornton - 2011 - Biochemistry and Molecular Biology Education - Wiley Online Library](https://iubmb.onlinelibrary.wiley.com/cms/asset/5a27afb8-d7ea-46c8-a4d0-c45ede7825bd/mfig001.jpg)
Real‐time PCR (qPCR) primer design using free online software - Thornton - 2011 - Biochemistry and Molecular Biology Education - Wiley Online Library
Designing PCR Primers using Primer3, UCSC in-Silico PCR and primer-BLAST Primers are short sequences of single stranded DNA that
![Positions of primers-OF (outer forward), F (forward), R (reverse), and... | Download Scientific Diagram Positions of primers-OF (outer forward), F (forward), R (reverse), and... | Download Scientific Diagram](https://www.researchgate.net/publication/14186676/figure/fig2/AS:667653278556168@1536192354575/Positions-of-primers-OF-outer-forward-F-forward-R-reverse-and-CR-control.png)